Journal: Journal of Inflammation Research

Article Title: Weighted Gene Co-Expression Network Analysis of Oxymatrine in Psoriasis Treatment

doi: 10.2147/JIR.S402535

Figure Lengend Snippet: List of Primers (5′ - 3′)

Article Snippet: The membranes were labeled with the following primary antibodies: β-actin (1:6000 dilution; Abcam, Cambridge, UK), S100A8 rabbit mAb (1:1000 dilution; Affinity Biosciences, USA), and SPRR2A mouse mAb (1:2000 dilution; Novus Biologicals, USA).

Techniques:

Journal: Journal of Inflammation Research

Article Title: Weighted Gene Co-Expression Network Analysis of Oxymatrine in Psoriasis Treatment

doi: 10.2147/JIR.S402535

Figure Lengend Snippet: Hub gene screening and verification. ( A ) Protein–protein interaction network of light-green module genes. The edge represents interactions between genes. A degree is used to describe the importance of the protein nodes in the network. ( B ) Immunohistochemical staining for CNFN and IOD values of CNFN. ( C ) Immunohistochemical staining for S100A8 IOD values of S100A8. ( D ) qRT-PCR of CNFN, S100A8, SPRR2A, SPRR2D , and SPRR2E mRNA expression. ( E ) Western blotting of S100A8, SPRR2A. ( F ) Schematic diagram of the relationship between EDC and psoriasis and oxymatrine. *vs normal group, * p < 0.05, ** p < 0.01, # vs before treatment, # p < 0.05, ## p < 0.01.

Article Snippet: The membranes were labeled with the following primary antibodies: β-actin (1:6000 dilution; Abcam, Cambridge, UK), S100A8 rabbit mAb (1:1000 dilution; Affinity Biosciences, USA), and SPRR2A mouse mAb (1:2000 dilution; Novus Biologicals, USA).

Techniques: Immunohistochemical staining, Staining, Quantitative RT-PCR, Expressing, Western Blot